12th ScienceChemistryHSC Projects

Vitamin C Content in Different Fruit Juices Project


Vitamin C, also known as L-ascorbate, is an essential nutrient vital for maintaining various bodily functions. This experiment aims to analyze and compare the vitamin C content in different fruit juices, providing a deeper understanding of the nutritional value of these beverages.

Importance of Vitamin C

Vitamin C plays a crucial role as an antioxidant, protecting the body against oxidative stress, and is involved in several enzymatic reactions. It is also critical for the maintenance of cartilage, bone, dentin, and blood vessels. Unlike many animals, humans cannot synthesize vitamin C, making dietary intake essential.


The goal is to determine which type of fruit juice provides the most vitamin C, using a laboratory titration method involving iodine and freshly squeezed or carton juices.

Materials and Methodology

  • Apparatus: Test tubes, syringes, beaker, mortar and pestle
  • Materials: Vitamin C tablets, DCPIP solution, various fruit juices, distilled water
  • Procedure:
    1. Prepare a standard solution of ascorbic acid by dissolving crushed vitamin C tablets in distilled water.
    2. Conduct a titration using the DCPIP solution and ascorbic acid to establish a standard curve.
    3. Test the vitamin C concentration in various fruit juices by titrating with DCPIP and recording the volume of juice required to decolorize the solution.

Results and Conclusion

The experiment concludes that fresh juices generally contain higher levels of vitamin C compared to carton juices. Among the tested samples, fresh lime juice exhibited the highest vitamin C content. This study provides valuable insights into the nutritional profiles of different fruit juices and affirms the hypothesis that fresher juices are richer in vitamin C.


These findings have significant implications for dietary choices, emphasizing the importance of consuming fresh fruits and their juices for optimal vitamin C intake.

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